Speaker
Description
Tuberculosis (TB) continues to represent a significant global health concern, impacting both humans and livestock. Mycobacterium bovis is the causative agent of bovine TB, an important zoonosis, whereas Mycobacterium tuberculosis, an organism adapted to humans, can occasionally cause symptomatic infection in cattle. The formation of granulomas, defined as organized multicellular tissue lesions, has been identified as a hallmark of TB in all affected host species. We developed a three-dimensional granuloma model, termed in vitro granuloma-like structure (IVGLS), using bovine leukocytes and magnetic nanoshuttles. Stable IVGLS were bioprinted to resemble either TB granulomas at innate stage, composed of macrophages, or the adaptive stage, containing also lymphocytes. M. bovis Bacillus Calmette-Guérin (BCG) replicated within the IVGLS, inducing foamy cell formation and triggering apoptosis particularly in the presence of lymphocytes. IVGLS released chemokines and Th1 cytokines and rewired their metabolism towards glycolysis. Cattle IVGLS recapitulate features of TB granulomas and facilitate spatial mapping of the immune responses.
Keywords
tuberculosis, mycobacteria, cattle, model, immunity, granuloma
| Registration ID | OHS25-152 |
|---|---|
| Professional Status of the Speaker | Professor |
| Junior Scientist Status | No, I am not a Junior Scientist. |
