Dr
Caroline Bost
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Climate change affects the distribution of vector-borne diseases. Orthonairoviruses are significant tick-borne pathogens affecting both humans and livestock. Serosurveillance is a critical tool for indirect detection of virus circulation, and has traditionally focused on anti-nucleoprotein (N) antibodies. However, the close antigenic relationship among orthonairoviruses N proteins often causes serological cross-reactivity, complicating accurate diagnosis. In this study, the glycoprotein GP38 was therefore evaluated as an alternative antigen.
We used field and experimental sera from the respective target species, i.e. humans and sheep, which were formerly infected with Crimean–Congo hemorrhagic fever virus (CCHFV) or Nairobi sheep disease virus (NSDV) to assess seroconversion and cross-reactivities between antigens of both viruses, first using an ELISA format. Additionally, both N and GP38 antigens were integrated into a multiplex bead-based assay for simultaneous detection and applied to screen field samples from small ruminants in CCHFV endemic and non-endemic regions. Confirmatory tests such as virus neutralization tests and immunofluorescence assays were used to validate the results.
The multiplex assay demonstrated high suitability for screening of large sample sets, providing similar detection rates as compared to ELISA methods. This approach offers a robust tool for future studies and surveillance of orthonairovirus infections.
Keywords
Orthonairovirus, Serology, Multiplex Assay, CCHFV, NSDV
| Registration ID |
OHS25-121
|
| Professional Status of the Speaker |
Postdoc
|
| Junior Scientist Status |
Yes, I am a Junior Scientist.
|
Dr
Caroline Bost
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Dr
Benjamin Gutjahr
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Ms
Mihiret H. Shimelis
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Ms
Elora Schlienger
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Ms
Franziska Neffgen
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Dr
Roland Suluku
(Animal Science, Serology and Molecular Laboratory, Njala University, Bo, Sierra Leone)
Dr
Simona Tchakarova
(Department for Exotic and Emerging Diseases, National Diagnostic and Research Veterinary Medical Institute, Bulgaria)
Prof.
Nazif Elaldi
(Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey)
Dr
Binnur Bagci
(Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey)
Dr
Ayse Nur Pektas
(Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey)
Prof.
Martin H. Groschup
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Dr
Anne Balkema Buschmann
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
Dr
Kerstin Fischer
(Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald – Riems Island)
There are no materials yet.
