International One Health Symposium 2025

Session

Session 8: Genomics & Proteomics

Oct 14, 2025, 1:45 PM

Room C4

Conveners

Session 8: Genomics & Proteomics

• Sébastien Calvignac-Spencer
• Torsten Semmler

243. Evolutionary Dynamics of Bluetongue virus Strains Circulating in Italy, 2024-2025

Gloria Plebani (Università degli Studi di Teramo, Istituto Zooprofilattico Sperimentale Abruzzo Molise-Teramo)

10/14/25, 1:45 PM

Genomics & Proteomics

Oral presentation

In 2024, Italy experienced a major resurgence of Bluetongue (BT), a viral disease of ruminants caused by Bluetongue virus (BTV), an RNA virus circulating in multiple serotypes transmitted by Culicoides midges. By April 2025, over 7,000 confirmed cases had been reported, mainly involving serotypes BTV-3, BTV-4, and BTV-8. We performed whole-genome sequencing (WGS) and phylogenetic analyses on...

307. Nanopore-Based Methylome Profiling of Microbial Communities

Valentina Galeone (RKI)

10/14/25, 2:00 PM

Genomics & Proteomics

Oral presentation

The methylome, defined as the complete set of genome-wide methylation marks, provides an additional layer of information beyond the genetic sequence, yet remains largely underexplored in bacterial genomics. Recent advances in Nanopore sequencing now allow investigation of DNA methylation not only in isolates but also at the community level, opening new opportunities to study epigenetic...

263. Combining Short- and Long-Read Sequencing to Investigate a Ralstonia pickettii Outbreak Linked to Contaminated Saline Solutions

Dr Vladimir Bajić (Genome Competence Centre (MF 1), Robert Koch Institute, Berlin, Germany)

10/14/25, 2:15 PM

Genomics & Proteomics

Oral presentation

In December 2023, the WHO reported a Ralstonia pickettii outbreak in Australia linked to contaminated saline solutions. Concurrently, German surveillance detected an increase in nosocomial R. pickettii bacteremia cases, prompting a national outbreak investigation.

From August 2023 to June 2024, 15 patient isolates were sequenced using Illumina whole genome sequencing. Five of these were...

297. Multi-omics investigation of L-sorbose metabolism in high-risk Escherichia coli lineages linking fitness and virulence

Lena-Sophie Swiatek

10/14/25, 2:30 PM

Genomics & Proteomics

Oral presentation

Certain sequence types (STs) of Escherichia coli, such as ST131 and ST648, belong to high-risk clonal lineages that pose serious public health threats by combining antimicrobial resistance (AMR), fitness, and virulence. In contrast, other subtypes e.g., ST10 are typically harmless and beneficial colonizers. To identify characteristics beyond AMR, we analyzed over 22,000 E. coli genomes and...

279. Automatic sequential clustering in the context of integrated genomic surveillance

Torsten Houwaart (Genome Competence Centre (MF1), Robert Koch Institute, Berlin, Germany)

10/14/25, 3:00 PM

Genomics & Proteomics

Oral presentation

Integrated Genomic Surveillance (IGS) enables the surveillance of various, public health-relevant bacterial and viral pathogens. For bacterial species, the identification of genomic clusters represents the foundation for outbreak investigations and epidemiological studies. Providing well-defined genomic clusters therefore represents an essential task of IGS, in order to support the...