Speaker
Description
Streptococcus canis (S. canis) is known as opportunistic pathogen colonizing dogs and cats, but also causes zoonotic diseases such as endocardites and septicaemiae in humans.
In the course of infection, S. canis enters the bloodstream and adheres to the endocardium as well as to the vasculature thereby inducing cell damage.
We aim to determine the impact of S. canis in particular on endothelial wound healing during cell culture infection. We used our established cell culture technique, which enables live cell imaging of the wound healing process after infection with streptococci. Additionally, differential immunofluorescence staining followed by confocal laser scanning microscopy was performed. Interestingly, incubation of endothelial cells (HUVEC) with S. canis causes cell damage and significantly inhibited endothelial gap closure.
With the aim to analyse the effect of S. canis infection on endothelial wound healing under physiological flow conditions present in the blood circulation, we combined the CSMA with a microfluidic system, which enables the application of defined shear stress values. Equally to infection under static conditions, circulating S. canis significantly inhibited endothelial gap closure at a defined shear stress.
The developed technique in combination with the microfluidic pump system proved to be ideally suited for the analysis of S. canis infection in the vascular system simulating systematic disease progression in vitro.
Keywords
Streptococcus canis, endothelium, cell migration, microfluidic, wound healing
| Professional Status of the Speaker | PhD Student |
|---|---|
| Junior Scientist Status | Yes, I am a Junior Scientist. |
| Registration-ID code | ZOO23-456 |
Primary author
External references
- 84
