Speaker
Description
Giardia duodenalis infects humans and a wide range of animal hosts; however, information on its genetic diversity in wildlife from the Brazilian Amazon remains scarce. This study investigated the genetic diversity and molecular epidemiology of G. duodenalis in humans and wild animals from the Amazon region using multilocus sequence typing (MLST). Assemblages A and B were identified in different host species. Genetic analyses revealed, for the first time, the occurrence of assemblage A in sloths and five bat species from the Amazon region. Assemblage A was also detected in non-human primates, an anteater (Tamandua tetradactyla), and humans. Sub-assemblage AII was identified exclusively in humans, whereas AI, a sub-assemblage with recognized zoonotic potential, was detected in sloths, bats, and an anteater. A novel genotype, designated AIV, was identified in non-human primates and formed a distinct lineage in multilocus phylogenetic analyses. Assemblage B exhibited higher genetic diversity than assemblage A and displayed a reticulated phylogenetic pattern. High-resolution MLST schemes based on six loci for assemblage A and three hypervariable loci for assemblage B provided lineage resolution comparable to genome-based analyses. These findings expand current knowledge of G. duodenalis diversity in the Brazilian Amazon and provide molecular data for epidemiological investigations at the human–wildlife interface within a One Health perspective.
Keywords
Assemblage; Sub-assemblage; MLST; pós-genômica; genotyping; zoonotic potential
| Registration ID | DE 315171187 |
|---|---|
| Professional Status of the Speaker | Postdoc |
| Junior Scientist Status | Yes, I am a Junior Scientist. |
Author
Co-authors
External references
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